Methylc capture sequencing. Bailey,8 Bernard Robaire,9 Sarah Kimmins,4,9 Maria C.
Methylc capture sequencing. Here, we present methylC-capture sequencing (MCC-Seq), a next-generation sequencing capture approach interrogating functional methylomes in disease-targeted tissues or cells. May 29, 2015 · AbstractMost genome-wide methylation studies (EWAS) of multifactorial disease traits use targeted arrays or enrichment methodologies preferentially covering CpG-dense regions, to characterize sufficiently large samples. , 2019 ) (Figure S5). . Methods: To characterize variation in sperm DNA methylation, we performed whole genome bisulfite sequencing (WGBS) on an equimolar pool of sperm DNA from a wide cross section of 30 men varying in age, fertility status, methylenetetrahydrofolate reductase (MTHFR) genotype, and exposures. San Gabriel,1,10 Armand Zini,1,10 Clifford Librach,11,12 Sergey Moskovtsev Among the current analysis techniques for DNA methylation, MethylC-capture sequencing (MCC-Seq) [4] based on NGS approach was developed for the targeted assessment of DNA methylation in a tissue-specific manner and successfully applied to targeting sperm epigenome [5 – 7]. The four most frequently used sequencing-based technologies are the bisulfite-based methods MethylC We confirmed by Spearman correlation analysis that the DNA methylation levels identified in the pooled sample were representative of the profiles of individual men obtained using our sperm-targeted methylC-capture sequencing (MCC-seq) (Chan et al. Customized MethylC-Capture Sequencing to Evaluate Variation in the Human Sperm DNA Methylome Representative of Altered Folate Metabolism Aug 8, 2019 · To this end, we recently implemented methylC-capture sequencing (MCC-Seq) for targeted assessment of DNA methylation in a tissue-specific manner (Allum et al. Capture of the biotinylated strand was performed with streptavidin-coated sepharose beads and washed using the PyroMark® Q24 Vacuum Workstation (Qiagen). 2015). Here, we used a human probe-designed MC-seq method to assay DNA methylation in DNA obtained from 13 CM and three AGM blood samples. May 29, 2015 · Here, we present methylC-capture sequencing (MCC-Seq), a next-generation sequencing capture approach interrogating functional methylomes in disease-targeted tissues or cells. Sep 19, 2010 · Analysis of DNA methylation patterns relies increasingly on sequencing-based profiling methods. Bailey,8 Bernard Robaire,9 Sarah Kimmins,4,9 Maria C. A sequencing primer was annealed to the isolated captured template strand and the pyrosequencing reaction was conducted using the PyroMark® Q24 kit (Qiagen) as per the manufacturer’s protocol. Feb 18, 2015 · This protocol describes how to prepare a sequencing library for MethylC-seq analysis, enabling the methylation status of cytosines in genomic DNA to be determined at nucleotide resolution on a Apr 18, 2018 · The recent development of methyl-capture sequencing (MC-seq) has an unprecedented advantage of cost-effectiveness, and further allows for extending the methylome coverage compared to conventional sequencing approaches. To overcome this limitation, we present here a new customizable, cost-effective approach, methylC-capture sequencing (MCC-Seq), for sequencing functional methylomes, while Customized MethylC-Capture Sequencing to Evaluate Variation in the Human Sperm DNA Methylome Representative of Altered Folate Metabolism Donovan Chan,1* Xiaojian Shao,2,3*† Marie-Charlotte Dumargne,1,4 Mahmoud Aarabi,5,6 Marie-Michelle Simon,7 Tony Kwan,7 Janice L. MethylC-capture sequencing (MCC-Seq) is an NGS capture approach that interrogates functional methylomes based on the NimbleGen SeqCap Epi CpGiant system with a unique design and long probes [26]. ludbxbx p1pf od0z tryjwz fg8hg7 hcnwvk y02cmne fdyxs s2bd mze